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allophycocyanin conjugated mouse monoclonal anti human axl antibody  (R&D Systems)


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    R&D Systems allophycocyanin conjugated mouse monoclonal anti human axl antibody
    Allophycocyanin Conjugated Mouse Monoclonal Anti Human Axl Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 44 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+monoclonal+anti+axl+antibody/Human+Axl+APC-conjugated+Antibody/pmc12455926-154-0-8
    Average 93 stars, based on 44 article reviews
    allophycocyanin conjugated mouse monoclonal anti human axl antibody - by Bioz Stars, 2026-09
    93/100 stars

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    Staining:

    Article Title: AXL Targeting Abrogates Autophagic Flux and Induces Immunogenic Cell Death in Drug-Resistant Cancer Cells
    Article Snippet: .. The cells were then stained with mouse monoclonal anti-AXL antibody (MAB154, R&D Systems, Minneapolis, Minnesota) for 30 minutes at room temperature, washed as above, and incubated with AF488-conjugated secondary goat anti-mouse antibody (A11029, Invitrogen) for 30 minutes at room temperature. .. The cells were washed and analyzed using a FACS Accuri C6 flow cytometer (BD Biosciences).

    Incubation:

    Article Title: AXL Targeting Abrogates Autophagic Flux and Induces Immunogenic Cell Death in Drug-Resistant Cancer Cells
    Article Snippet: .. The cells were then stained with mouse monoclonal anti-AXL antibody (MAB154, R&D Systems, Minneapolis, Minnesota) for 30 minutes at room temperature, washed as above, and incubated with AF488-conjugated secondary goat anti-mouse antibody (A11029, Invitrogen) for 30 minutes at room temperature. .. The cells were washed and analyzed using a FACS Accuri C6 flow cytometer (BD Biosciences).



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    Analyses of Tlr13 −/− , Rnaset2 −/− , and Rnaset2 −/− Tlr13 −/− mice. (A) Survival curve of wild-type mice (black) and Rnaset2 −/− mice (red). (B) Schematic representation of Tlr13 gene targeting. The filled and open boxes represent coding and 3′-untranslated regions of the Tlr13 gene, respectively. Neo indicates the neomycin resistance gene: B, BamH I; and E, EcoR I. (C) PCR analyses with primers indicated in B to determine genotypes of indicated mice. (D) Production of IL-6 and CCL5 by BM-derived macrophages left unstimulated or stimulated with indicated TLR ligands. Results represent mean values with SD from triplicates. (E) Hb concentration, hematocrit, and mean corpuscular volume of RBCs in indicated mice ( n = 8–16). (F) Percentage of IL-6 + cells in splenic Ly6C low or Ly6C hi macrophages from wild-type mice with or without antibiotics treatment after in vitro stimulation with the TLR13 ligand Sa19 (5 µg/ml) or the TLR4 ligand lipid A (1 µg/ml) together with Brefeldin A for 3 h ( n = 3). (G) Immunoblotting of whole cell lysates from wild-type and Rnaset2 −/− Ba/F3 with anti-RNase T2 <t>mAb.</t> β-actin was also immunoprobed to show that the amounts of samples are equal. (H) Ba/F3 expressing Tlr3, Tlr7, or Tlr13-HA tag were subjected to membrane-permeabilized staining with <t>mAbs</t> to TLR3 (PaT3), TLR7 (A94B10), TLR13 (M13), and αHA. (I and J) The gating strategy of splenic cells (I) and hepatic cells (J). (K) Serum titer of autoantibodies to SSA, Sm, and dsDNAs in indicated mice ( n = 8–26). (L) The numbers of CD11b + Ly6C hi and CD11b + Ly6C low macrophages in the spleens of indicated mice ( n = 5–8). *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001. Source data are available for this figure: .
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    Analyses of Tlr13 −/− , Rnaset2 −/− , and Rnaset2 −/− Tlr13 −/− mice. (A) Survival curve of wild-type mice (black) and Rnaset2 −/− mice (red). (B) Schematic representation of Tlr13 gene targeting. The filled and open boxes represent coding and 3′-untranslated regions of the Tlr13 gene, respectively. Neo indicates the neomycin resistance gene: B, BamH I; and E, EcoR I. (C) PCR analyses with primers indicated in B to determine genotypes of indicated mice. (D) Production of IL-6 and CCL5 by BM-derived macrophages left unstimulated or stimulated with indicated TLR ligands. Results represent mean values with SD from triplicates. (E) Hb concentration, hematocrit, and mean corpuscular volume of RBCs in indicated mice ( n = 8–16). (F) Percentage of IL-6 + cells in splenic Ly6C low or Ly6C hi macrophages from wild-type mice with or without antibiotics treatment after in vitro stimulation with the TLR13 ligand Sa19 (5 µg/ml) or the TLR4 ligand lipid A (1 µg/ml) together with Brefeldin A for 3 h ( n = 3). (G) Immunoblotting of whole cell lysates from wild-type and Rnaset2 −/− Ba/F3 with anti-RNase T2 <t>mAb.</t> β-actin was also immunoprobed to show that the amounts of samples are equal. (H) Ba/F3 expressing Tlr3, Tlr7, or Tlr13-HA tag were subjected to membrane-permeabilized staining with <t>mAbs</t> to TLR3 (PaT3), TLR7 (A94B10), TLR13 (M13), and αHA. (I and J) The gating strategy of splenic cells (I) and hepatic cells (J). (K) Serum titer of autoantibodies to SSA, Sm, and dsDNAs in indicated mice ( n = 8–26). (L) The numbers of CD11b + Ly6C hi and CD11b + Ly6C low macrophages in the spleens of indicated mice ( n = 5–8). *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001. Source data are available for this figure: .
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    Analyses of Tlr13 −/− , Rnaset2 −/− , and Rnaset2 −/− Tlr13 −/− mice. (A) Survival curve of wild-type mice (black) and Rnaset2 −/− mice (red). (B) Schematic representation of Tlr13 gene targeting. The filled and open boxes represent coding and 3′-untranslated regions of the Tlr13 gene, respectively. Neo indicates the neomycin resistance gene: B, BamH I; and E, EcoR I. (C) PCR analyses with primers indicated in B to determine genotypes of indicated mice. (D) Production of IL-6 and CCL5 by BM-derived macrophages left unstimulated or stimulated with indicated TLR ligands. Results represent mean values with SD from triplicates. (E) Hb concentration, hematocrit, and mean corpuscular volume of RBCs in indicated mice ( n = 8–16). (F) Percentage of IL-6 + cells in splenic Ly6C low or Ly6C hi macrophages from wild-type mice with or without antibiotics treatment after in vitro stimulation with the TLR13 ligand Sa19 (5 µg/ml) or the TLR4 ligand lipid A (1 µg/ml) together with Brefeldin A for 3 h ( n = 3). (G) Immunoblotting of whole cell lysates from wild-type and Rnaset2 −/− Ba/F3 with anti-RNase T2 <t>mAb.</t> β-actin was also immunoprobed to show that the amounts of samples are equal. (H) Ba/F3 expressing Tlr3, Tlr7, or Tlr13-HA tag were subjected to membrane-permeabilized staining with <t>mAbs</t> to TLR3 (PaT3), TLR7 (A94B10), TLR13 (M13), and αHA. (I and J) The gating strategy of splenic cells (I) and hepatic cells (J). (K) Serum titer of autoantibodies to SSA, Sm, and dsDNAs in indicated mice ( n = 8–26). (L) The numbers of CD11b + Ly6C hi and CD11b + Ly6C low macrophages in the spleens of indicated mice ( n = 5–8). *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001. Source data are available for this figure: .
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    Analyses of Tlr13 −/− , Rnaset2 −/− , and Rnaset2 −/− Tlr13 −/− mice. (A) Survival curve of wild-type mice (black) and Rnaset2 −/− mice (red). (B) Schematic representation of Tlr13 gene targeting. The filled and open boxes represent coding and 3′-untranslated regions of the Tlr13 gene, respectively. Neo indicates the neomycin resistance gene: B, BamH I; and E, EcoR I. (C) PCR analyses with primers indicated in B to determine genotypes of indicated mice. (D) Production of IL-6 and CCL5 by BM-derived macrophages left unstimulated or stimulated with indicated TLR ligands. Results represent mean values with SD from triplicates. (E) Hb concentration, hematocrit, and mean corpuscular volume of RBCs in indicated mice ( n = 8–16). (F) Percentage of IL-6 + cells in splenic Ly6C low or Ly6C hi macrophages from wild-type mice with or without antibiotics treatment after in vitro stimulation with the TLR13 ligand Sa19 (5 µg/ml) or the TLR4 ligand lipid A (1 µg/ml) together with Brefeldin A for 3 h ( n = 3). (G) Immunoblotting of whole cell lysates from wild-type and Rnaset2 −/− Ba/F3 with anti-RNase T2 <t>mAb.</t> β-actin was also immunoprobed to show that the amounts of samples are equal. (H) Ba/F3 expressing Tlr3, Tlr7, or Tlr13-HA tag were subjected to membrane-permeabilized staining with <t>mAbs</t> to TLR3 (PaT3), TLR7 (A94B10), TLR13 (M13), and αHA. (I and J) The gating strategy of splenic cells (I) and hepatic cells (J). (K) Serum titer of autoantibodies to SSA, Sm, and dsDNAs in indicated mice ( n = 8–26). (L) The numbers of CD11b + Ly6C hi and CD11b + Ly6C low macrophages in the spleens of indicated mice ( n = 5–8). *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001. Source data are available for this figure: .
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    Analyses of Tlr13 −/− , Rnaset2 −/− , and Rnaset2 −/− Tlr13 −/− mice. (A) Survival curve of wild-type mice (black) and Rnaset2 −/− mice (red). (B) Schematic representation of Tlr13 gene targeting. The filled and open boxes represent coding and 3′-untranslated regions of the Tlr13 gene, respectively. Neo indicates the neomycin resistance gene: B, BamH I; and E, EcoR I. (C) PCR analyses with primers indicated in B to determine genotypes of indicated mice. (D) Production of IL-6 and CCL5 by BM-derived macrophages left unstimulated or stimulated with indicated TLR ligands. Results represent mean values with SD from triplicates. (E) Hb concentration, hematocrit, and mean corpuscular volume of RBCs in indicated mice ( n = 8–16). (F) Percentage of IL-6 + cells in splenic Ly6C low or Ly6C hi macrophages from wild-type mice with or without antibiotics treatment after in vitro stimulation with the TLR13 ligand Sa19 (5 µg/ml) or the TLR4 ligand lipid A (1 µg/ml) together with Brefeldin A for 3 h ( n = 3). (G) Immunoblotting of whole cell lysates from wild-type and Rnaset2 −/− Ba/F3 with anti-RNase T2 mAb. β-actin was also immunoprobed to show that the amounts of samples are equal. (H) Ba/F3 expressing Tlr3, Tlr7, or Tlr13-HA tag were subjected to membrane-permeabilized staining with mAbs to TLR3 (PaT3), TLR7 (A94B10), TLR13 (M13), and αHA. (I and J) The gating strategy of splenic cells (I) and hepatic cells (J). (K) Serum titer of autoantibodies to SSA, Sm, and dsDNAs in indicated mice ( n = 8–26). (L) The numbers of CD11b + Ly6C hi and CD11b + Ly6C low macrophages in the spleens of indicated mice ( n = 5–8). *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001. Source data are available for this figure: .

    Journal: The Journal of Experimental Medicine

    Article Title: RNase T2 deficiency promotes TLR13-dependent replenishment of tissue-protective Kupffer cells

    doi: 10.1084/jem.20230647

    Figure Lengend Snippet: Analyses of Tlr13 −/− , Rnaset2 −/− , and Rnaset2 −/− Tlr13 −/− mice. (A) Survival curve of wild-type mice (black) and Rnaset2 −/− mice (red). (B) Schematic representation of Tlr13 gene targeting. The filled and open boxes represent coding and 3′-untranslated regions of the Tlr13 gene, respectively. Neo indicates the neomycin resistance gene: B, BamH I; and E, EcoR I. (C) PCR analyses with primers indicated in B to determine genotypes of indicated mice. (D) Production of IL-6 and CCL5 by BM-derived macrophages left unstimulated or stimulated with indicated TLR ligands. Results represent mean values with SD from triplicates. (E) Hb concentration, hematocrit, and mean corpuscular volume of RBCs in indicated mice ( n = 8–16). (F) Percentage of IL-6 + cells in splenic Ly6C low or Ly6C hi macrophages from wild-type mice with or without antibiotics treatment after in vitro stimulation with the TLR13 ligand Sa19 (5 µg/ml) or the TLR4 ligand lipid A (1 µg/ml) together with Brefeldin A for 3 h ( n = 3). (G) Immunoblotting of whole cell lysates from wild-type and Rnaset2 −/− Ba/F3 with anti-RNase T2 mAb. β-actin was also immunoprobed to show that the amounts of samples are equal. (H) Ba/F3 expressing Tlr3, Tlr7, or Tlr13-HA tag were subjected to membrane-permeabilized staining with mAbs to TLR3 (PaT3), TLR7 (A94B10), TLR13 (M13), and αHA. (I and J) The gating strategy of splenic cells (I) and hepatic cells (J). (K) Serum titer of autoantibodies to SSA, Sm, and dsDNAs in indicated mice ( n = 8–26). (L) The numbers of CD11b + Ly6C hi and CD11b + Ly6C low macrophages in the spleens of indicated mice ( n = 5–8). *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001. Source data are available for this figure: .

    Article Snippet: Monoclonal anti-mouse CD4 (clone RM4-5) antibody was purchased from Invitrogen and monoclonal anti-mouse Axl antibody (clone 175128) from R&D Systems.

    Techniques: Derivative Assay, Concentration Assay, In Vitro, Western Blot, Expressing, Membrane, Staining

    TLR13-dependent macrophage accumulation in the spleen and liver. (A and B) TLR13 expression in indicated immune cells in the spleen (A) and liver (B) of indicated mice. Red and black histograms show staining with anti-TLR13 mAb and isotype-matched antibody, respectively. (C and D) The cell numbers of indicated immune cells in the spleen from indicated mice ( n = 5–8). (E and H) The percentages of indicated immune cells in the spleen and liver of wild-type and Rt2 −/− mice. (F and G) The cell numbers of indicated immune cells in the liver in indicated mice ( n = 5–8). (I) The cell numbers of splenic or hepatic macrophages in indicated mice with or without antibiotics treatment ( n = 4). **P < 0.01, ***P < 0.001 and ****P < 0.0001.

    Journal: The Journal of Experimental Medicine

    Article Title: RNase T2 deficiency promotes TLR13-dependent replenishment of tissue-protective Kupffer cells

    doi: 10.1084/jem.20230647

    Figure Lengend Snippet: TLR13-dependent macrophage accumulation in the spleen and liver. (A and B) TLR13 expression in indicated immune cells in the spleen (A) and liver (B) of indicated mice. Red and black histograms show staining with anti-TLR13 mAb and isotype-matched antibody, respectively. (C and D) The cell numbers of indicated immune cells in the spleen from indicated mice ( n = 5–8). (E and H) The percentages of indicated immune cells in the spleen and liver of wild-type and Rt2 −/− mice. (F and G) The cell numbers of indicated immune cells in the liver in indicated mice ( n = 5–8). (I) The cell numbers of splenic or hepatic macrophages in indicated mice with or without antibiotics treatment ( n = 4). **P < 0.01, ***P < 0.001 and ****P < 0.0001.

    Article Snippet: Monoclonal anti-mouse CD4 (clone RM4-5) antibody was purchased from Invitrogen and monoclonal anti-mouse Axl antibody (clone 175128) from R&D Systems.

    Techniques: Expressing, Staining

    TLR13 responses in various organs of Rnaset2 −/− . (A) Gating strategy to detect microglia, Ly6C hi , and Ly6C low macrophages in the brains of indicated mice. (B) The percentages of F4/80 + CD45.2 int microglia, Ly6C hi , and Ly6C lo macrophage in CD45 + cells from the brain of indicated mice ( n = 4). (C, F, and I) TLR13 expression in indicated immune cells in the brain, lung, and kidney of indicated mice. Red and black histograms show staining with anti-TLR13 mAb and isotype-matched antibody, respectively. (D) Gating strategy to detect alveolar, Ly6C hi, and Ly6C low macrophages from the lungs of indicated mice. (E) The percentages of F4/80 + CD11b int alveolar, Ly6C hi , and Ly6C low macrophage in CD45 + cells from the lungs of indicated mice ( n = 4). (G) Gating strategy to detect Ly6C hi and Ly6C low macrophages from the kidneys of indicated mice. (H) The percentages of Ly6C hi and Ly6C low macrophages in CD45 + cells from the kidney of indicated mice ( n = 3). (J) The percentages of the EdU + cells in indicated macrophage subsets from the indicated organs of indicated mice. *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001.

    Journal: The Journal of Experimental Medicine

    Article Title: RNase T2 deficiency promotes TLR13-dependent replenishment of tissue-protective Kupffer cells

    doi: 10.1084/jem.20230647

    Figure Lengend Snippet: TLR13 responses in various organs of Rnaset2 −/− . (A) Gating strategy to detect microglia, Ly6C hi , and Ly6C low macrophages in the brains of indicated mice. (B) The percentages of F4/80 + CD45.2 int microglia, Ly6C hi , and Ly6C lo macrophage in CD45 + cells from the brain of indicated mice ( n = 4). (C, F, and I) TLR13 expression in indicated immune cells in the brain, lung, and kidney of indicated mice. Red and black histograms show staining with anti-TLR13 mAb and isotype-matched antibody, respectively. (D) Gating strategy to detect alveolar, Ly6C hi, and Ly6C low macrophages from the lungs of indicated mice. (E) The percentages of F4/80 + CD11b int alveolar, Ly6C hi , and Ly6C low macrophage in CD45 + cells from the lungs of indicated mice ( n = 4). (G) Gating strategy to detect Ly6C hi and Ly6C low macrophages from the kidneys of indicated mice. (H) The percentages of Ly6C hi and Ly6C low macrophages in CD45 + cells from the kidney of indicated mice ( n = 3). (J) The percentages of the EdU + cells in indicated macrophage subsets from the indicated organs of indicated mice. *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001.

    Article Snippet: Monoclonal anti-mouse CD4 (clone RM4-5) antibody was purchased from Invitrogen and monoclonal anti-mouse Axl antibody (clone 175128) from R&D Systems.

    Techniques: Expressing, Staining